During the process of catabolizing starch, optimum temperature levels are vital in heightening the reliability of the enzymes used. In this case, two enzymes were used to determine the ideal optimum temperature for starch to catabolize. The first one was Bacterial amylase. This bacteria is also called Bacillus licheniform. The second enzyme used was Fungal amylase, or famously known as Aspergillus oryzae. The core objective of using these different enzymes was to help determine the best temperature that would favor the starch into catabolizing.
An interval of 0-10 minutes was used to further the test of the different enzymes. The temperatures used were 0, 25, 55, and 85 degrees Celsius. The purpose of this stage in the experiment was to measure the efficiency of each amylase in breaking down the starch. As the results indicated, it was vital to use fungal and bacterial amylase to help the researchers understand which enzyme slowed the catabolizing process down right from the beginning.
Record the duration that enzymes took to facilitate the starch process
Later on, a spot plate was brought in to record the duration that the enzymes took to facilitate the starch process, in light with the best pleasant temperature. For this reason, the data collected was recorded in minutes alongside degrees Celsius to represent the results gathered from the optimum temperature range. Using the different four temperature scales that were mentioned in the previous section of this study, an equilibrium of a 5-minute duration was directed at each slot of time of the spottable. Finally, the horizontal row got filled with three drops of iodine.
The starch was combined with the fungal amylase that was as a result of careful filtration of the different temperature elements. In this case, a toothpick and a pipette were used during the temperature retrieval process, as well as the starch combination. After the first result, as opposed to repeating the procedure using bacterial amylase, a fungal amylase was used. The main intention was to determine how the effect of fungal and bacterial amylase is bound to differ according to the enzyme used. Most importantly, it is essential to keep a note of the fact that the amylase tubes were not changed, despite switching the fungal and bacterial amylase.
Finally, three drops of iodine were used to all temperature slots. At an interval of ten minutes, the data was collected, and a little fungal amylase was combined to show the relativity of the temperature slots. The role of the iodine was to facilitate the functionality of the enzymes to determine the best optimum temperature range for the starch under experiment to catabolize.
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